Oral Presentation 23rd International Society of Magnetic Resonance Conference 2023

Unlocking micro-scale NMR: an affordable, versatile microchip-based NMR system for high-impact applications (#85)

Marco Grisi 1 , Kathryn M Marable 1 , Giulia M Sivelli 1 , Guillaume M Gruet 1 , Gaurasundar Marc Conley 1
  1. Annaida Technologies SA, Lausanne, VAUD, Switzerland

Magnetic Resonance Spectroscopy (MRS) is a powerful, non-invasive method with significant potential in fundamental research, drug development, personalized medicine, and beyond [1-3]. Micro-NMR, an emerging field within NMR, has increased sensitivity and reduced sample volume demands, paving the way for new applications in portable NMR and mass-limited biological samples [4-7]. We present a novel, scalable, and user-friendly micro-NMR probe system featuring a single-chip transceiver, microcoil, and 3D micro-printing [8-9]. This system can be used with standard NMR spectrometers for measurements of samples with volumes between 10 nL and 100 pL at frequencies between 150 MHz and 600 MHz. The CMOS-based design enables simultaneous acquisition from multiple sensor coils on the same device, and additive manufacturing allows customization for various magnets, spectrometer systems, and applications. This presentation will discuss the system architecture, features, and performance, as well as prospects for future developments. We demonstrate the probe's utility in nanoliter-size human 3D cell cultures, early mammalian embryos and oocytes, highlighting its potential for disease monitoring in microscopic cell cultures.

 

 

 

 

 

 

 

 

 

  1. De Graaf, Robin A. In vivo NMR spectroscopy: principles and techniques. John Wiley & Sons, 2019.
  2. Damadian, Raymond. "Tumor detection by nuclear magnetic resonance." Science 171.3976 (1971): 1151-1153.
  3. Le Bihan, Denis, et al. "MR imaging of intravoxel incoherent motions: application to diffusion and perfusion in neurologic disorders." Radiology 161.2 (1986): 401-407.
  4. Grisi, Marco, et al. "NMR spectroscopy of single sub-nL ova with inductive ultra-compact single-chip probes." Scientific reports 7.1 (2017): 1-8.
  5. Montinaro, E., et al. "3D printed microchannels for sub-nL NMR spectroscopy." PloS one 13.5 (2018): e0192780.
  6. F. Dreyer, Q. Yang, D. Krüger and J. Anders, "A chip-based NMR relaxometry system for point-of-care analysis," 2022 IEEE Biomedical Circuits and Systems Conference (BioCAS), 2022, pp. 183-187, doi: 10.1109/BioCAS54905.2022.9948623.
  7. Patra, B., Sharma, M., Hale, W. et al. Time-resolved non-invasive metabolomic monitoring of a single cancer spheroid by microfluidic NMR. Sci Rep 11, 53 (2021).
  8. Sivelli, Giulia, et al. "NMR spectroscopy of a single mammalian early stage embryo." Journal of Magnetic Resonance 335 (2022): 107142.
  9. Grisi, Marco, et al. "NMR microsystem for label-free characterization of 3D nanoliter microtissues." Scientific reports 10.1 (2020): 1-9.