Proton nuclear magnetic resonance (NMR) N-acetyl signals (Glyc) from glycoproteins and supramolecular phospholipids composite peak (SPC) from phospholipid quaternary nitrogen methyls in lipoprotein particles can give important systemic metabolic information in serum, but their absolute quantification is compromised by heavy overlap in NMR spectra. We present a J-Edited DIffusional (JEDI) NMR spectroscopic approach to selectively augment signals from the inflammatory marker peaks Glyc and SPCs in blood serum NMR spectra, which enables direct integration of peaks associated with molecules found in specific compartments. The JEDI method was developed and optimized on high field 600 MHz systems but can be effectively translated to low field (80 MHz) benchtop NMR systems without added time constraints due to lower sensitivity of the benchtop system. This research shows that the inflammatory biomarkers Glyc and SPC can be measured effectively on benchtop NMR instruments that do not require housing in a complex laboratory environment, thus lowering the barrier to clinical translation of this diagnostic technology.